Coding
Part:BBa_K2220038:Design
Designed by: Nan Rong Group: iGEM17_BNU-China (2017-10-14)
Flagellin fusion with eGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1491
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 301
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
we had done codon optimization, for better expression in S.cerevisiae
Source
eGFP gene comes from team Tsinghua. flagellin gene FliC comes from E.coli K12